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Fig. 3. Expression of sglA gene during growth and development. Ax4 wild-type cells were allowed to develop synchronously on filters. Total RNA samples were prepared at the indicated time points and analyzed for sglA mRNA levels. The band intensities on the northern blots were quantified with a FUJIFILM FLA-2000 PhosphorImager. The mRNA levels are depicted as -fold increase over the mRNA level at 0 hours of development.