Fig. 8. Tricaine treatment phenocopies the effect of the mao mutation on Rohon-Beard cell death. (A) In lateral views of control (top) and tricaine-treated (bottom) embryos, HuA immunocytochemistry reveals dorsal root ganglia (asterisks). In addition, RB cells (arrows) are revealed in the tricaine-treated but not the control embryo. Scale bar: 40 µm. (B) RB cell counts indicate that there is a significant difference in the number of RB neurons present in 72 hpf tricaine-treated and control embryos (P< 0.000001; tricaine treated, n=9; untreated, n=10). Tricaine-treatment suppresses the normal loss of RB cells, as does the mao mutation.