Fig. 2. The Cs-lhx3 gene product of Ciona savignyi. (A) The predicted amino acid sequence. The LIM domains are shown by italic capitals and homeodomain is underlined. (B) Relationship of Cs-LHX3 with other LIM-homeodomain proteins. The phylogenetic tree of the homeodomains of the LIM-homeodomain proteins was constructed using the neighbor-joining method. The homeodomains of Cs-OTX and Cs-TTF1 were used as an outgroup. Branch length is proportional to the number of amino acid substitution; the scale bar indicates 0.1 amino acid substitution per position in the sequence.