Fig. 6. Effects of functional suppression with morpholino oligos of Cs-otx (D-F) and Cs-ttf1 (G-I) or overexpression of Cs-ttf1 (J-L) on the endodermal cell differentiation. (A-C) Larvae developed from eggs injected with lacZ morpholino (control). (A) Histochemical staining of AP in the endodermal cells (arrows) of the larval trunk. In Ciona larvae, AP is also expressed B-line notochord cells (arrowhead). (B,C) Larvae that show normal development of two sensory organs, otolith (arrow) and ocellus (arrowhead), which is evident by pigment cell development. (D-F) Larvae developed from eggs injected with Cs-otx morpholino. (D) Histochemical staining of AP. (E,F) Larvae that show a failure of development of two sensory organs. The development of the anterior head is also suppressed by Cs-otx morpholino, causing this region to appear smooth. (G-I) Larvae that developed from eggs injected with Cs-ttf1 morpholino. (G) Histochemical staining of AP activity. (H,I) Larvae that show normal development of two sensory organs, otolith (arrow) and ocellus (arrowhead). (J-L) Larvae developed from eggs injected with Cs-ttf1 mRNA. Histochemical staining of AP activity in tailbud embryos (J) and in 110-cell arrested embryo. Ventral view (K) and anterior view (L). Scale bars: in A, 100 µm for A,D,G,J; in B, 100 µm for B,E,H; in C, 100 µm for C,F,I; and in K, 100 µm for K,L.