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Fig. 1. The expression pattern of (A) Hnf3ß, (B) Chrd, (C) GsclacZ, (D) Cer1 and (E) Otx2 in the ES, MS, LS and the EB stage embryos. Gene expression was revealed by whole-mount mRNA in situ hybridization (A,B,D,E); by immunostaining (A, asterisk); and by X-gal staining for ß-galactosidase activity (C). In (B), ES stage embryo, which displays no Chrd activity, is not shown and combined in situ hybridization of T and Chrd are for EB stage embryo. Scale bar: 300 µm.