Fig. 2. Temporal pattern of Kermit expression. (A) Northern blot of Kermit expression. Kermit was detected as a single band at 4.7 kb. Lanes 1 to 3: total RNA (20 µg) from oocytes, stage 9, and stage 11 embryos. (B) RT-PCR analyses of Kermit, Xfz3, Xfz7 and Xfz8 expression. Lanes 1 to 3: stage II, III-IV and V-VI oocytes. Lanes 4 to 11: RNA from stage 3-4, 7, 10, 12, 20, 27, 32 and 40 embryos. Lane 12: negative control without reverse transcriptase. FGFR1 was used as a loading control.