Fig. 3. Spatial pattern of Kermit expression. In situ hybridization was performed at early gastrula, early and late neurula, and tadpoles stages. (A) A stage 10+ gastrula, with the dorsal lip visible in the lower left. (B) A hemisection of a stage 14 neurulae, with dorsal oriented upwards; diffuse staining is visible in the neural plate, notochord, and paraxial mesoderm. (C) Strong staining in the anterior neural plate of a stage 18 neurula (anterior is towards the left). (D,E) Stage 26 and (F) stage 32 tadpoles, with kermit expression in the neural tube, eye, otic vesicle and branchial arches. (G) Expression of Xfz3 in a stage 32 tadpole.