Fig. 6. Xfz3 causes Kermit membrane translocation. Kermit-GFP or control GFP mRNA were injected into animal poles with Xfz3 or Xfz8 mRNA at the one-cell stage. Animal caps were dissected at stage 8, fixed and analyzed by confocal microscopy. (A) Kermit-GFP is localized to both cytoplasm and nucleus, similar to the GFP control (compare A with C). (B) Expression of Xfz3 causes a marked translocation of Kermit to the plasma membrane, with little remaining in the nucleus or cytoplasm. (C) Control expression of GFP, with localization in cytoplasm and nucleus. (D) Xfz3 expression has no effect on the distribution of GFP alone. (E) Xfz8 has only a small effect on Kermit subcellular distribution (compare A with E).