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Fig. 3. Expression of lacZ in Halocynthia tailbud-stage embryos that developed from eggs injected with various deletion constructs of pAs-T2/lacZ. (A) Injection of p(-2164)As-T2/lacZ resulted in the expression of the reporter gene in muscle cells (Mu; arrow) and TT cells (arrowhead). En, endoderm; N, notochord. Scale bar: 100 µm. (B) Injection of p(-2164 ~ -555)As-T2/lacZ resulted in the expression of lacZ in TT (tip of the tail) cells. (C) Injection of p(-230)As-T2/lacZ resulted in the expression of lacZ in muscle cells (Mu).