Fig. 4. Characterisation of anti-B-type cyclin antisense oligonucleotides. (A) Sequence alignment of oligonucleotide ‘cyc8’ with different B-type cyclins. Mismatches are shown in red. (B) Effects of oligonucleotides ‘cyc8’ and anti B5-2 on cyclin synthesis monitored by the indicated immunoprecipitates of [³⁵S]methionine-labelled egg extracts. (C) Northern blot analysis of mRNA isolated from oocytes which were, uninjected (C), injected with a mixture of 75 ng ‘cyc8’ and 25 ng of anti B5-2 (AS) or injected with the same oligonucleotides in the sense orientation (S).