Fig. 2. Analysis of droplet spreads of embryo cell suspensions. (A,B) Droplet spreads of suspensions from whole wild-type (A) and Igf2^+m/-p (B) embryos stained with DAPI and visualised with fluorescence microscopy. Mitotic figures (arrow in A) and pyknotic cells with less than 2C DNA content (arrows in B) can be identified. (C) Similarity in the distribution of fluorescence emitted per nuclei from litter-matched whole embryo spreads, (wild type (blue) n=436 nuclei, Igf2^+m/-p (red) n=499 nuclei). (D) Significantly increased pyknotic cell number in Igf2^+m/-p cell spreads were observed (**P=0.012 Student’s t-test, wild type (blue) n=2341 nuclei from seven embryos, Igf2^+m/-p (red) n=1277 nuclei from seven embryos). No significant differences (NS) were detected for mitotic cell counts (P=0.47). Scale bars: 10 µm in A,B.