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Fig. 8. Cell size analysis of unfixed cells in suspension. (A) Red blood cell (mouse) size distribution profiles following incubation in buffers of different osmolarity (insert) using a Coulter multi-sizer. (B) Red blood cell changes in cell size with osmolarity, from A. (C) Multiple cell diameter profiles from unfixed whole wild-type (blue, n=5) and Igf2+m/-p (red, n=6) litter-matched embryos, aged E9.5 using cell number of a fixed wild-type embryo.