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Fig. 8. Elevation of screw activity does not rescue a sogYSO6 mutant. (A) Lateral view of a stage 13 sogYSO6; P[Kr-lacZ] embryo after dorsal injection of 2.7 µg/µl of scw mRNA embryo. No ß-galactosidase staining is observed, indicating that the overexpression of scw mRNA does not restore amnioserosa in sog embryos (0%; n=38). (B) Lateral view of a Df(2L)OD16 P[Kr-lacZ] stage 13 embryo lacking scw, stained for ß-galactosidase activity after injection of 27 ng/µl of scw mRNA. While uninjected scw mutants fail to develop amnioserosa, injection of 27 ng/µl of scw mRNA resulted in restoration of amnioserosa (83%; n=34).