Fig. 3. Sensitivity to lithium of mbl heterozygotes as compared to wildtypes. Eye phenotype frequencies in 2 dpf zebrafish embryos treated at 4 hpf with lithium (0.3 M for 5-10 minutes). (A) Heterozygous mbl^+/- x HLwt outcrosses. A 6-minute treatment resulted in 50% eyeless and 50% wild-type embryos. (B) Wild-type crosses where a 6-minute treatment resulted in a less than 2% phenotype. Numbers of embryos pooled from 2 independent experiments are indicated at the top of the graph. Wt, wild type; se, small eyes; ne, no eyes; de, dead.