Fig. 5. IFE-1::GFP is associated with P granules in wild-type (N2) but not in pgl-1 embryos. The genotypes of embryos are listed above the columns. Embryos measure 30x50 µm. (A,E) Fluorescence micrographs of 16-20-cell stage embryos. IFE-1::GFP is localized in perinuclear granules in the germline cell (P₄) in N2 but not in pgl-1. (H) Fluorescence micrograph of a four-cell stage embryo. IFE-1::GFP signal is not detected in the cytoplasm or P granules of RNAi embryos. (B-D,F,G) Immunofluorescence micrographs of 70-90-cell stage embryos co-stained with anti-GFP (B,F) and anti-GLH-2 (C,G) antibodies. Anti-GFP staining is detectable in perinuclear granules in the germ cells (Z₂ and Z₃) of N2 but not in pgl-1 germ cells. (D) The merged image of B,C demonstrates double staining of P granules by anti-GFP and anti-GLH-2 antibodies in N2.