Fig. 1. Expression of Sox10 protein in neural crest stem cells and peripheral glia. Neural crest stem cells (NCSCs) were replated from neural crest explants, fixed after 4 hours, and immunolabeled using anti-p75 antibody (visualized by Cy3 fluorescence) (A) and anti-Sox10 antibody (visualized by FITC fluorescence) (B). Note that virtually all freshly isolated NCSCs are double-positive for p75 and Sox10. Sister cultures of replated NCSCs were allowed to develop in conditions (Materials and Methods) that predominantly generate S100-positive glia (D), neurofilament160 (NF)-positive neurons (G) or smooth muscle actin (SMA)-positive non-neural cells (J). Double-labeling of the cultures for Sox10 revealed that Sox10 expression is maintained in the glial lineage (E) while it is downregulated in neuronal (H) and non-neural (K) cells. (C,F,I,L) Corresponding phase-contrast pictures.