Fig. 3. Loss of glial potential in Sox10^–/– undifferentiated neural crest cells. (A-J) Cells isolated from DRG of wild-type or Sox10^–/– mutant E13 embryos were either incubated in differentiation medium (‘no add’) or in differentiation medium supplemented with NRG1 (‘+NRG1’). In both conditions, surviving undifferentiated Sox10^–/– mutant p75-positive cells (C,D) were not able to produce any O4-positive glia (F,G) but adopted the morphology of smooth muscle-like non-neural cells (I,J). Wild-type cells generated mainly O4-positive glia cells (E). (K) Quantitative clonal analysis of undifferentiated cells incubated in the presence of NRG1. G indicates clones containing exclusively glial cells; G/NN, clones containing glia and non-neural smooth muscle-like cells; NN, clones containing exclusively non-neural smooth muscle-like cells. For the quantitative analysis, expression of p75, NF160 and absence of these markers were used to distinguish between different fates. Numbers are given as percentage of all founder cells originally plated. Note that the indicated numbers of clone phenotypes do not sum to 100% because of cell death of a proportion of the founder cells (see Fig. 2). The data are expressed as mean±s.d. of four independent experiments. 60 clones were scored per experiment.