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Fig. 2. Essentially correct Alx3/lacZ expression in knock-in mice; comparative expression analysis of Alx3 and Alx4 in E10.5 embryos. (A) Detection of Alx3 mRNA (whole-mount in situ hybridisation) in whole embryo. (B) lacZ expression in Alx3+/- mouse embryo. Expression of Alx3 and lacZ are qualitatively identical, confirming proper targeting of the Alx3 locus. Staining in brain vesicles in A is background staining. (C,D) Radioactive in situ hybridisation showing overlapping expression of Alx3 and Alx4 in frontonasal processes (FNP) at E9.5. (G,H) Expression of Alx3 and Alx4 in medial nasal process (MNP) and mandibular arch (BA) at E10.5. (E,I) Schemes indicating approximate orientation of sections. (F,J) Whole-mount in situ hybridisation of E10.5 embryos showing overlap of expression in cranium and anterior mesenchyme of limbs.