Fig. 5. Craniofacial abnormalities in Alx3/Alx4^lst-J double mutant newborn mice. Alizarin Red (bone)/Alcian Blue (cartilage) stained skulls are shown. First column shows a dorsal view on the vaults of the skulls. Second column shows dorsal views on skulls from which vaults were dissected. Third column shows a ventral view on skulls from which mandibles and vaults were dissected. Fourth column shows the dissected mandibles. Genotypes are indicated to the left. Green arrows indicate the basipresphenoid, which constitutes the border of neural crest-derived skeletal elements and cephalic and somitic mesoderm derived skeletal elements. White arrows indicate the nasal septum.