Fig. 3. Necab coexpresses with Pax6 in the olfactory placodes, the iris and the ciliary margin of the retina. (A-D) Radioactive in situ hybridization at 10.5 dpc (coronal sections) showing co-expression of Necab and Pax6 in the retinal-pigmented epithelium (rpe), the neural retina (nr), the olfactory placodes (op), the forebrain (fb) and the pre-optic area (poa). Necab is also expressed in the dorsal portion of the midbrain (A, arrows). Arrowhead in B indicates the lens pit. (E,F) Necab is expressed in the dorsal thalamus (dt), the roof plate of the midbrain (rp), and at low level in the hypothalamus (ht), (coronal sections, 13.5 dpc). (G,H) Necab and Pax6 expression is detected in the distal portion of the retina (cm) and in the future iris (white arrowhead). The black arrowhead indicates the cornea. (I) Necab is expressed in the ciliary margin (cm) and iris (17.5 dpc), and at low level in the ganglion cells layer (gc). (J,K) Necab and Pax6 are co-expressed in the ciliary margin and in the iris (white arrowhead) (17.5 dpc). ls, lens; vt, ventral thalamus.