Fig. 6. Results of experiment C (see Fig. 1E). (A-C) Dorsal views of embryos hybridized in whole mount with Pax1 (A), Pax3 (B) and MyoD (C) probes, one day after the surgery (E2.5). (D) 2.5 days later (i.e. E5), the side deprived of midline cells is very short compared to the contralateral side, which developed normally and became U-shaped. (E-G) Serial sections at E2.5 hybridized with Shh (E), Pax1/Pax6 (F) probes or examined for apoptosis using TUNEL (arrowheads) (G). DM, dermomyotome; En, endoderm; FP, floor plate; NT, neural tube; No, notochord; Scl, sclerotome; *, ink.