Fig. 3. Phenotypic rescue of el-mutant embryos. Uninjected (A,B) and injected (C,D) wild-type (B) and mutant embryos (A,C,D) raised for 8 days at restrictive temperature. Dorsal views, anterior is towards the top. Note dark pigmented melanophores in wild-type embryo (arrowhead in B), lacking in mutants (A,C,D). (A) No eyes form in mutants. (C,D) Rescued eye formation in mutant embryos injected with Rx3 plasmid. (C) Note complete rescue of both eyes. (E) Table displaying results of different DNA and control injections. N, number of injected embryos; el (b/b) homozygous mutant embryos as judged by absence of dark melanophores. No rescue is observed in control injected embryos.