Fig. 4. The expression pattern of clk-2. (A) Northern and western analyses of clk-2 at all developmental stages (E, embryos; L1-L4, larval stages; A, young adults; glp-4(bn2ts) adults). (B) clk-2 mRNA and CLK-2 protein levels in mutant backgrounds glp-4(bn2ts), fem-2(b245ts) and fem-3(q20ts) at 25°C. (C) CLK-2 protein levels in the wild type and clk-2(qm37) mutants at three temperatures. For northerns, actin is used as a loading control; for westerns, equal loading was confirmed by Coomassie Blue staining of identical gels (not shown). As expected, the clk-2 band detected in the northerns is at 2.8 kb, and the CLK-2 band detected in westerns is at 100 kDa.