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Fig. 5. Subcellular localization of a functional CLK-2::GFP fusion. Bright fluorescence is found in the cytoplasm of a variety of cells, and appears excluded from their nuclei (arrows). (A) Neurons in ganglia of the head. (B) Three neurons in the pre-anal ganglion. (C) A distal tip cell and its projections (arrowheads). (D) A two-cell embryo. Only one nucleus is in the plane of focus, but fluorescence was observed to be extranuclear in the second cell as well. (E) A region of the hypodermis. Scale bars: ~10 µm.