Fig. 7. Glia undergo extra rounds of DNA replication in ttk mutant embryos. After BrdU incorporation, wild-type (A) and ttk1e11 mutant embryos (B) were stained with anti-Repo (red) and anti-BrdU (green) antibodies. In wild-type stage 12 embryos, four Repo-positive longitudinal glia in each hemi-neuromere incorporate BrdU. By contrast, six to seven glia undergo DNA replication in mutant embryos of the equivalent stage. The number of Repo-positive glia associated with the longitudinal connectives is increased from 9.9±0.76 in wild-type embryos (C) to 17.8±2.23 in ttk mutant embryos (D). Data are mean±s.d. of 20 determinations. Anterior is upwards; open arrows, midline.