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Fig. 2. gcm mRNA is not asymmetrically localized during NGB 6-4T cell division. Ventral view gcm mRNA localization in the NGB 6-4T lineage; oriented as in Fig. 1. NGB 6-4T (6-4T) and the G daughter cell are circled (yellow dots); the adjacent NB 6-2 (6-2), NB 7-2 (7-2), NGB 7-4 (7-4) and the lateral glial precursor (GP) are labeled when visible and were used as landmarks (see Materials and Methods). (A) gcm mRNA is expressed in the predivisional NGB 6-4T (6-4T). (B) Anti-phosphohistone H3 (purple) is a marker for mitotic DNA. gcm mRNA (green) is equally distributed throughout the cell in the mitotic NGB 6-4T. (C) gcm mRNA is present in both the apical NGB 6-4T and its basally positioned G daughter cell after mitosis. (D) After the medial migration of the G daughter cell, gcm mRNA is detected in the G daughter cell but not in the post-divisional NGB 6-4T.