Fig. 4. prospero and miranda, but not numb or staufen, are required for glial development in the NGB 6-4T lineage. Ventral view of a stage 13 embryos showing Repo-positive glia derived from the NGB 6-4T lineage. Three thoracic segments are shown in all panels; anterior is upwards; n
20 for all. (A) Wild-type glial development in the NGB 6-4T lineage. Left: a summary showing the positions of glia (red dots) derived from NGB 6-4T (purple circle) with migration patterns (lines). Right: wild-type embryo stained for Repo, brackets indicate NGB6-4T-derived glial progeny. (B,C) numb and staufen mutants show a wild-type pattern of NGB 6-4T-derived glia. (D) prospero mutants show a loss of NGB 6-4T-derived glia. Repo-positive cells in the region of 6-4T glia in prospero mutants are not derived from NGB 6-4T, based on their lack of expression of the NGB 6-4T marker eagle (data not shown, see also Fig. 5). (E) miranda mutants have a variable phenotype showing reduced, normal or extra NGB 6-4T-derived glia (see text for details).