Fig. 6. Miranda is required to prevent gcm upregulation in NGB 6-4T and NGB 7-4 lineages. (A) Wild type and miranda mutant embryos stained for Gcm protein. Anterior is upwards; midline is towards the left. Stage 12 embryos are shown for NGB 6-4T, late stage 11 embryos are shown for NGB 7-4. In wild-type embryos (top row), Gcm is not detected in NGB 6-4T or NGB 7-4 (dotted circles). In miranda mutant embryos (bottom row), Gcm protein is present in NGB 6-4T and NGB 7-4 (dotted circles; 60% of hemisegments scored for both; n=91 hemisegments). (B) miranda mutant embryos can make extra glia from NGB 6-4T. Mutant embryos were stained with antibodies to Repo (to identify glia; red), Eg (to visualize the entire NGB 6-4T lineage, blue) and En (as a second marker for NGB 6-4T, green). Arrowheads, NGB 6-4T; arrows, NGB 6-4T-derived glial progeny; n=38 hemisegments. One segment is shown, the midline is indicated by the vertical bar. To the left of the midline, the NGB 6-4T shows high levels of Repo expression and no neuronal progeny were produced by this NGB. By contrast, to the right of the midline the NGB 6-4T is not expressing Repo and several neuronal progeny have been produced (out of plane of focus).