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Fig. 5. cls mutants lack nac and spa expression. Lateral views of caudal trunk of 25 hpf wild-type and cls embryos after in situ hybridisation with nac (A,B), spa (E,F) and dct (G,H) probes. (C,D) nac/mitf expression is decreased weakly (C) or strongly (D) after injection with either a low or high dose, respectively, of sox10 morpholino. n, neural tube; y, yolk sac. Scale bar: 75 µm.