Fig. 5. Rescue of the spg mutant phenotype by pou2 mRNA injection. Wild-type and spgm793 mutant embryos were injected at the one-cell stage with pou2 mRNA, fixed at the five- to six-somite stage (A-C) or at 24 hpf (D-I), and assayed for the expression of pax2.1 (A-C), pax2.1 and krx20 (D-F), or wnt1 (G-I). All embryos shown were genotyped by allele-specific PCR. Arrows indicate the position of the MHB. Orientation: (A-C) dorsal views, anterior at the top; (D-I) lateral views, anterior towards the left.