Fig. 1. Recombinant XWnt8 protein posteriorizes neuralized animal cap cells in a dose-dependent manner. (A) Experimental design. RT-PCR analyses were carried out when control embryos reached neural tube stage (stage 20). (B) RT-PCR analysis of whole embryos (we), intact control animal caps (co) and caps that were dissociated and reaggregated (diss.) as depicted in A and treated during dissociation with 0, 2 nM or 20 nM recombinant XWnt8. (C) RT-PCR analysis of whole embryos (we), intact animal caps (co) and caps that have been dissociated and reaggregated as depicted in A, and treated during dissociation with 25 nM XWnt8 (+) in the absence (–) or presence (+) of recombinant MFz8-CRD. H4, histone4 for normalization; –RT, negative control without reverse transcriptase.