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Fig. 4. Immature Bergmann glia are indistinguishable between double null mutants and controls at p1. Immunostaining using nestin (A,B) and RC2 (C,D) antibodies shows that immature double null mutant Bergmann protrusions (B,D) do not differ from immature control protrusions (A,C) at P1. Arrows point to positively staining protrusions. PS, pial surface; PC, Purkinje cell layer. Scale bars: 20 µm.