Fig. 1. Rst overexpression and mutant phenotypes in the stage 16 abdominal muscle pattern. Three dimensional reconstructions of confocal stacks of antibody staining against ß-3-tubulin. Anterior is to the left and dorsal is upwards. (A) Heatshocked yw embryo. Arrowheads indicate ventral acute (va) and ventral oblique (vo) muscles. (B) Heatshocked pCa18Z3.1 embryo. Muscles are thin and some are missing (e.g. ventral acute muscles). (C) Heatshocked yw embryo. Single confocal plane interior to the muscles. (D) Heatshocked pCa18Z3.1 1 embryo. Single confocal plane as in C, showing a large amount of unfused fusion-competent myoblasts. (E) da-Gal4/+;UAS-rst/+ embryo showing unfused fusion-competent myoblasts (asterisk), thin muscles and some muscles with ectopic projections (arrow). Several muscles are missing. lt, lateral transversal muscle. (F) rst^irreC1 mutant embryo showing missing and thin muscles (arrowheads). (G) rst⁶ mutant embryo with misled (arrow) and thin muscles (arrowhead). Asterisks mark unfused fusion-competent myoblasts. Scale bars: 20 µm.