Fig. 2. The kirre and rst genes and proteins. (A) Physical map of the kirre locus. The first exon of kirre resides about 30 kb distal and is not depicted. Unbroken lines symbolize genomic DNA present in the depicted deficiency chromosomes and the cosP479BE transgene. Hatched bars refer to the closest possible breakpoint predictions. Arrowheads symbolize kirre-specific primers used in single fly PCR. Open reading frames are depicted as black bars and orientation of transcription by arrows. Restriction sites: B, BglII; E, EcoRI; H, HindIII; S, SacI; Xb, XbaI; Xh, XhoI. (B) Schematic comparison of the Rst and Kirre proteins. Numbers refer to sizes of Immunoglobulin (Ig) domains and to percentages of sequence identities of paralogous Ig domains, respectively. Arrows indicate the serine- and glycine-rich repeats of Kirre and Rst, respectively. An asterisk marks the sequence stretch separating the autophosphorylation domain and the PDZ-binding motif in Kirre. (C) Alignment of Rst, Kirre, Sns and Hibris. Residues identical in Rst and Kirre are on green background, residues identical within all four sequences are boxed in red. Borders of Ig domains (Ramos et al., 1993) are marked by a vertical bar and an inverted triangle. Arrows indicate cysteines involved in forming a disulphide bond. Serine- and glycine-rich repeats of Kirre and Rst, respectively, are underlined. Putative phosphorylation sites conserved within Kirre and Rst are marked by P in an inverted triangle. Unconserved sites are boxed. APD, autophosphorylation domain with consensus sequence below; IC, intracellular domain; IG, Immunoglobulin domain; PADVI, conserved motif; opa, opa-like repeat; PDZ, PDZ-binding motif; SP, signal peptide; TM, transmembrane domain. Boxed sequence stretches contain the corresponding patterns.