Development -- Strünkelnberg et al. 128 (21): 4229 Figure IG5

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Fig. 5. kirre is not essential for muscle development. (A) Schematic depiction of the white-Notch region and deficiencies used in this study (see text). Df(1)w67c23 is a viable deletion removing 3C2-5 (Lefevre and Green, 1972) (see discussion). (B) Single fly PCR proves that kirre is not present in the genome of Df(1)N^54l9/Y;cosP479BE/+ flies. Lanes 1,3,5: kirre-specific primers. Lanes 2,4,6: rst-specific primers. Lanes 1,2: Df(1)N^54l9/Y;cosP479BE/+ as template. Lanes 3,4: wild-type Berlin males as template. Lanes 5,6: no template. (C) Abdominal muscle pattern of Df(1)N^54l9/Y;cosP479BE/+ embryo stained for muscle Myosin is indistinguishable from wild type. Anterior is towards the left, dorsal is upwards. Scale bar: 20 µm.