Fig. 2. Effects of Shh protein and antibody administration on mesenchymal gene expression in the GT. GT explants were micro-dissected from 11.5 dpc embryos, implanted with Shh protein (s) and control BSA (b) beads, and cultured for 24 hours. (A-D) The expression of Ptch1 (A), Bmp4 (B), Hoxd13 (C) and Fgf10 (D) were augmented in regions adjacent to the Shh protein bead. Arrowheads and arrows indicate the augmented and endogenous expression, respectively. (E,F) Mesenchymal (endogenous) expression of Ptch1 in the GT explant was reduced after treatment with an anti-Shh neutralizing antibody for 24 hours. Scale bar: 250 µm in A-D; 312.5 µm in E,F.