Fig. 5. Immunoblot analysis of the transgenic plants. (A) Levels of the COP9 signalosome subunits in the CSN3 transgenic plants. Proteins were extracted from the defective organs (as indicated on top) from various transgenic lines (TG). Antibodies against CSN3, CSN6 and CSN1 were used to detect the COP9 signalosome level (marked on the right). The corresponding wild-type tissues (WT) were used as a control. Rpn6 was used to control equal loading. (B) Levels of ubiquitinated proteins in the transgenic plants and fus11-U203 mutant. The total protein extracts were separated on 12% SDS page and detected with antibodies against ubiquitin. J. Leaves, juvenile leaves; A. Leaves, adult leaves. The juvenile leaves, adult leaves and flower I were from lines S3-C-A, S3-C-A and S3-C-C, respectively of the T₂ generation in the Columbia background. The transgenic seedling and flower II were from line S3-L-D of the T₃ generation in the Landsberg background. 8-day-old fus11-U203 seedlings were used as a control. Many other transgenic lines were examined and similar results were observed.