Fig. 3. Vascular and floral defects in hemizygous 826 plants. (A) Abnormal venation pattern in a cotyledon of a plant hemizygous for the 826 T-DNA. Note that the central vein is short and a secondary vein is interrupted. (B) Vascular pattern complexity in the most advanced cotyledon of wild-type (GUS negative, white bars) and hemizygous 826 (GUS positive, black bars) seedlings. The y-axis shows the percentage of seedlings falling into the categories represented on the x-axis. (C) Hemizygous 826 flowers carry only four to five stamens, as clearly shown in a cross-section of a flower bud from an 826 hemizygous plant. (D) The floral defect originates from the floral meristem, as indicated by the absence of one vascular strand (compare top with bottom arrow) in a cross-section through the receptacle of a young flower.