Fig. 4. Modulation of the suHw function by the regulatory context is independent of interacting promoters. Transgenic embryos containing AE1 between divergently transcribed eve-Scr promoter/reporter genes are hybridized with anti-reporter probes (A-D, see Fig. 2 legend for general descriptions). (A,B) AE1 activates eve/CAT expression (a, CAT expression) but not the Scr/lacZ expression (B, lacZ expression) in transgenic embryos. (C,D) AE1-eve/CAT interaction can only be partially blocked by the intervening suHw insulator (C, CAT expression) without concomitant restoration of AE1-Scr/lacZ interaction (D, lacZ expression). The insets show the internal control Scr expression (see Fig. 2 legend, and Methods for details).