Fig. 3. Time-dependent effect of olomoucine and amino-purvalanol on germination. Olomoucine (100 µM) or amino-purvalanol (5 µM) were added at various times AF (as indicated on the x-axis) and the proportion of germinated zygotes was scored 36 hours later (red and white triangles, repectively). To determine the percentage of cells in G2 phase at the time of drug addition (red squares), aphidicolin (20 µM) was added at various times AF (x-axis) and the proportion of divided cells was scored 36 hours later. Photopolarisation (black circles) and axis fixation (white circles) are reported for each time point. This graph is representative of three independent experiments.