Fig. 8. Comparison of time courses of changes in cGMP (A,D), cADPR (B,E) and IP₃ (C,F) contents of LY83583 preincubated eggs with those of unincubated control eggs after insemination. A. crassispina eggs for LY83583-preincubation (black symbols) and control (white symbols) (A-C) were prepared from the same batch of sea urchins and H. pulcherrimus eggs for both experiments (D-F) were obtained from the same batch of eggs, and each set of eggs was processed in parallel. Data on H. pulcherrimus control eggs are the same with Fig. 6B. Note the inhibition of cADPR increase for the first minute in LY83583-preincubated eggs of both species. The reason for the cADPR spikes at 70 and 150 seconds in the presence of LY83583 is unknown.