Fig. 9. Averaged time courses of the changes in cGMP (A), IP₃ (B) contents and [Ca²⁺]_i (C) in LY83583 preincubated A. crassispina eggs. cGMP and IP₃ contents are averages of four independent experiments and [Ca²⁺]_i data are averages of five and nine experiments for control and 200 µM LY83583, respectively. Error bars represent s.d. An arrow in C indicates the onset of fertilization potential. Asterisks indicate the points showing significant difference between LY83583 preincubated eggs (black symbols) and unincubated control eggs (white symbols) (P<0.05, t-test). Note almost no increase in cGMP and the diminution both of IP₃ increase and Ca²⁺ transients.