Fig. 1. Gliolectin is expressed in the embryonic Drosophila nerve cord during commissural and longitudinal pathfinding. Expression of gliolectin in the midline glia at early stage 13. (A) In wild-type embryos, the midline glia stain with the anti-Gliolectin monoclonal antibody 1B7 (arrowhead, ventral view of a whole mount embryo). (B) A wild-type embryo dissected after staining with 1B7 (black) and BP102 (brown) demonstrates the spatial and temporal relation between Gliolectin expression and axon scaffold formation at early stage 13. (C) In 3013 homozygotes, Gliolectin expression is completely absent at the midline by 1B7 antibody staining (arrowhead). Scale bar: 64 µm in A; 25 µm in B; 16 µm in C.