Fig. 1. Ap protein levels differ between the dorsal wing pouch and the hinge region. (A) ap mRNA levels visualized in a wild-type disc by in situ hybridization with an ap antisense RNA probe. (B) Third instar wing imaginal disc labeled with anti-Ap. Ap levels were higher in the hinge region than in the dorsal wing pouch. The difference in Ap protein levels between pouch and hinge is not reflected by a difference in transcript levels. (C) Anti-dLMO staining of the disc in B. (D) Overlay of B and C. Low Ap levels coincide with high dLMO levels.