Fig. 2. dLMO decreases Ap levels in the dorsal wing pouch. (A) Wing disc with a clone of dLMO³⁹ mutant cells. dLMO protein was not detectable in the mutant clone (center). Ap levels were higher in the dLMO mutant cells (left ). Overlay of Ap and dLMO staining (right). (B) Region of an ap-lacZ wing disc with a large clone of dLMO³⁹ mutant cells (arrow). The clone was labeled by the absence of GFP (right). Ap protein increased in the clone (left), but ap-lacZ transcript levels reflected by anti-ß-gal did not differ between the clone and the surrounding tissue (center). (C) Disc with clones of dLMO-expressing cells (labeled by co-expression of GFP, green). The reduction of Ap is most obvious in the hinge where the endogenous protein level is higher (long arrow). dLMO also reduced Ap levels in the wing pouch (note the loss of nuclear label in the clone near the short arrow). The residual signal in the clone reflects nonspecific background produced by the antibody (as in the ventral compartment in B).