Fig. 3. Ap is degraded by a proteasome-mediated mechanism in the wing pouch. (A,B) Wild-type, DMSO-treated discs stained with anti-Ap (A) and anti-Ci (B) antibodies. (C,D) Wild-type discs treated with MG-132 for 3 hours. Ap staining was more intense in the dorsal wing pouch after treatment with the proteasome-inhibitor (D) when compared with the hinge (C). In D, the previously reported stabilization of Ci is evident. (E) Ratio of Ap staining intensity in the pouch and hinge region (averaged over three areas of the wing pouch in the discs in A,C). The level of Ap in the pouch and hinge are more similar in the MG132-treated disc.