Fig. 4. L1 and TAG-1 immunopositive axons are both found in the intermediate zone. Double immunostaining against TAG-1 (green, A) and L1 (red, B) in cryostat sections of E14.5 mouse brain. (C) Superimposed image of A and B. No differences in the expression pattern of L1 are detected in cortical slices cultured in the presence of antibodies against TAG-1 (E) compared with control ones (D). Scale bar: 100 µm.