Fig. 4. Comparison of OOC-5, OOC-3 and HDEL staining in wild-type embryos. Confocal micrographs of wild-type embryos double labeled with anti-OOC-5 (A,D,G,J) or anti-OOC-3 (M,P,S) and anti-HDEL antibodies (B,E,H,K,N,Q,T). Merged images showing the extent of signal overlap (yellow) are shown in C,F,I,L,O,R,U. (A-L) Co-localization of OOC-5 and HDEL. (A-C) One-cell prophase embryo with one pronucleus visible; the other pronucleus is out of the plane of focus. (D-F) One-cell embryo in metaphase. (G-I) Two-cell in which AB (left cell) is in anaphase and P1 is in metaphase. (J-L) Four-cell embryo in which the AB cells (top left cells) are in prophase and EMS and P2 are in interphase. (M-U) Co-localization of OOC-3 and HDEL staining. (M-O) One-cell embryo in anaphase. (P-R) Two-cell embryo in early interphase. (S-U) Two-cell embryo in which AB is in anaphase and P1 is in metaphase. The stage of the cell cycle was determined by DAPI staining (not shown) in all cases. Scale bar: 10 µm.