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Fig. 2. EphA4 protein marks the surfaces of muscle precursors. High magnification views of proximal limb mesoderm from early stage 17 embryo, stained with annexin, EphA4 (green) and Pax7 (red) antibodies. (A) Many muscle precursors possess EphA4 protein on their surfaces (arrows in A-C). (B) Annexin labeling reveals cell surfaces of all cells in forelimb. (C) Merged image of A,B, with annexin labeling in blue.