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Fig. 4. Biochemical characterization of selected pelle mutations. The pelle alleles that were assayed in this experiment are indicated across the top. wt, wild type. (Top panel) Phosphorylation of Tube by Pelle mutant proteins. (Middle panel) Autophosphorylation of Pelle mutant proteins. (Bottom panel) Pelle protein levels in the indicated mutant backgrounds, as assayed by immunoblotting.